Anti-IL1 beta Antibody A00101-3 from BosterBio

Product Specs
ItemAnti-IL1 beta Antibody
CompanyBosterBio
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberA00101-3
ApplicationsIF,IHC,WB
FormatLyophilized, 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
ReactivityMouse
HostRabbit
Brand/TrademarkBosterbio
NCBI Full Gene Nameinterleukin 1 beta
NCBI Gene AliasesIL-1beta, Il-1b
ClonalityPolyclonal
Quantity100µg
Concentration0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure
Research AreaAtherosclerosis, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Metabolism, Obesity, Vascular Inflammation
StorageStore Anti-IL-1 beta antibody at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening
IsotypeIgG
ImmunogenThis antibody was prepared by repeated immunizations with recombinant mouse IL-1ß produced in E.coli. The MW of recombinant mouse IL-1ß was 17 kDa
PurityThis is an IgG preparation of whole rabbit serum purified by DEAE fractionation. This antibody is primarily directed against mature, 17,000 MW mouse IL-1ß and is useful in determining its presence in various assays. The antibody does not recognize human IL-1ß or mouse IL-1α based on a neutralization assay. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1ß. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1ß containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1ß precursor molecule. Denatured 31,000 precursor IL-1ß will be recognized by this antibody
TargetIL1B